Effects of centrifugation treatment before electroporation on gene editing in pig embryos.

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作者： Bin,Liu ^[1] ; Manita,Wittayarat ^[2] ; Koki,Takebayashi ^[1] ; Qingyi,Lin ^[1] ; Nanaka,Torigoe ^[1] ; Zhao,Namula ^[3] ; Maki,Hirata ^[1] ; Megumi,Nagahara ^[1] ; Fuminori,Tanihara ^[4] ; Takeshige,Otoi ^[5]

作者单位： Bio-Innovation Research Center, Tokushima University, Tokushima, 7793233, Japan.;Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, 7793233, Japan. ^[1] Faculty of Veterinary Science, Prince of Songkla University, Songkhla, 90110, Thailand. ^[2] Bio-Innovation Research Center, Tokushima University, Tokushima, 7793233, Japan.;College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, China. ^[3] Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, 7793233, Japan. ^[4] Bio-Innovation Research Center, Tokushima University, Tokushima, 7793233, Japan. otoi@tokushima-u.ac.jp.;Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, 7793233, Japan. otoi@tokushima-u.ac.jp.;Laboratory of Animal Reproduction, Bio-Innovation Research Center, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima, 779-3233, Japan. otoi@tokushima-u.ac.jp. ^[5]

关键词 CRISPR/Cas9 system Centrifugation Electroporation Mosaic mutation Zona pellucida