An in vitro platform for quantifying cell cycle phase lengths in primary human intestinal epithelial cells.

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第一作者： Michael J,Cotton

第一单位： Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, 27599, USA.

作者： Michael J,Cotton ^[1] ; Pablo,Ariel ^[2] ; Kaiwen,Chen ^[1] ; Vanessa A,Walcott ^[3] ; Michelle,Dixit ^[1] ; Keith A,Breau ^[3] ; Caroline M,Hinesley ^[4] ; Katarzyna M,Kedziora ^[5] ; Cynthia Y,Tang ^[6] ; Anna,Zheng ^[3] ; Scott T,Magness ^[7] ; Joseph,Burclaff ^[8]

作者单位： Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, 27599, USA. ^[1] Microscopy Services Laboratory, Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. ^[2] Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. ^[3] Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. ^[4] Department of Cell Biology, Center for Biologic Imaging (CBI), University of Pittsburgh, Pittsburgh, PA, 15260, USA. ^[5] School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. ^[6] Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, 27599, USA. scott_magness@med.unc.edu.;Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. scott_magness@med.unc.edu.;Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. scott_magness@med.unc.edu. ^[7] Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, 27599, USA. joseph.burclaff@med.unc.edu.;Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. joseph.burclaff@med.unc.edu. ^[8]

关键词 Cell cycle phase Collagen press Fluorescent reporter Intestinal stem cell Live imaging analysis

主题词人类(Humans);上皮细胞(Epithelial Cells);肠黏膜(Intestinal Mucosa);细胞周期(Cell Cycle);寡霉素类(Oligomycins);细胞, 培养的(Cells, Cultured)

DOI 10.1038/s41598-024-66042-9

PMID 38956443

发布时间 2024-07-15

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准确一般字义偏差语意偏离译文作用小提交