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Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics.

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第一作者： Louis,Delhaye

第一单位： VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium; Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium; OncoRNALab, Center for Medical Genetics Ghent (CMGG), Ghent University, Ghent, Belgium.

作者： Louis,Delhaye ^[1] ; George D,Moschonas ^[2] ; Daria,Fijalkowska ^[3] ; Annick,Verhee ^[3] ; Delphine,De Sutter ^[3] ; Tessa,Van de Steene ^[3] ; Margaux,De Meyer ^[3] ; Hanna,Grzesik ^[4] ; Laura,Van Moortel ^[5] ; Karolien,De Bosscher ^[5] ; Thomas,Jacobs ^[6] ; Sven,Eyckerman ^[7]

作者单位： VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium; Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium; OncoRNALab, Center for Medical Genetics Ghent (CMGG), Ghent University, Ghent, Belgium. ^[1] VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium; Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium. ^[2] VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium; Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium. ^[3] VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium; Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Department of Cellular and Molecular Medicine, Faculty of Medicine, KU Leuven, Leuven, Belgium. ^[4] VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium; Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium. ^[5] VIB-UGent Center for Plant Systems Biology, VIB, Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Faculty of Sciences, Ghent University, Ghent, Belgium. ^[6] VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium; Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium. Electronic address: sven.eyckerman@ugent.vib.be. ^[7]

关键词 CP: Biotechnology Golden Gate assembly T2A peptide T2A split/link TurboID glucocorticoid receptor interactomics non-structural protein 7 nucleocapsid protein proteomics proximity labeling