A precise and sustainable doxycycline-inducible cell line development platform for reliable mammalian cell engineering with gain-of-function mutations.

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第一作者： Sung Wook,Shin

第一单位： Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea.

作者： Sung Wook,Shin ^[1] ; Honggi,Min ^[1] ; Jiwon,Kim ^[1] ; Jae Seong,Lee ^[2]

作者单位： Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea. ^[1] Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea; Advanced College of Bio-convergence Engineering, Ajou University, Suwon, 16499, Republic of Korea. Electronic address: jaeseonglee@ajou.ac.kr. ^[2]

关键词 Cell line development Epigenetic silencing Gain-of-function mutations Mammalian synthetic biology Site-specific integration Tet-On3G inducible system

主题词多西环素(Doxycycline);人类(Humans);HEK293细胞(HEK293 Cells);细胞系(Cell Line);动物(Animals)

DOI 10.1016/j.ymben.2024.09.004

PMID 39242074

发布时间 2024-12-02

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