NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals.

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作者： Nikolaos,Mougios ^[1] ; Elena R,Cotroneo ^[2] ; Nils,Imse ^[2] ; Jonas,Setzke ^[3] ; Silvio O,Rizzoli ^[4] ; Nadja A,Simeth ^[5] ; Roman,Tsukanov ^[6] ; Felipe,Opazo ^[7]

作者单位： Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.;Center for Biostructural Imaging of Neurodegeneration (BIN), University of Göttingen Medical Center, Göttingen, Germany. ^[1] Institute for Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, Germany. ^[2] Center for Biostructural Imaging of Neurodegeneration (BIN), University of Göttingen Medical Center, Göttingen, Germany. ^[3] Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.;Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen, Göttingen, Germany. ^[4] Institute for Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, Germany.;Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen, Göttingen, Germany. ^[5] III. Institute of Physics - Biophysics, Georg August University, Göttingen, Germany. ^[6] Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany. fopazo@gwdg.de.;Center for Biostructural Imaging of Neurodegeneration (BIN), University of Göttingen Medical Center, Göttingen, Germany. fopazo@gwdg.de.;NanoTag Biotechnologies GmbH, Göttingen, Germany. fopazo@gwdg.de. ^[7]

主题词显微镜检查, 荧光(Microscopy, Fluorescence);人类(Humans);显微镜检查, 共焦(Microscopy, Confocal);动物(Animals);荧光染料(Fluorescent Dyes)

DOI 10.1038/s41467-024-53030-w

PMID 39384781

发布时间 2024-10-13

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