Use of stable isotope-labeled fatty acids to measure desaturase activities with negative chemical ionization GC-MS.

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第一作者： Youenn,Launay

第一单位： Institut Agro, INSERM, INRAE, Univ Rennes, NuMeCan, Rennes 35000, France; INSERM, INRAE, Univ Rennes, NuMeCan, Rennes 35000, France.

作者： Youenn,Launay ^[1] ; Iwan,Jan ^[2] ; Vincent,Ciesielski ^[1] ; Lydie,Hue ^[2] ; Mélodie,Succar ^[2] ; Léa,Fret ^[2] ; Thomas,Guerbette ^[1] ; Karima,Begriche ^[3] ; Philippe,Legrand ^[1] ; Daniel,Catheline ^[1] ; Manuel,Vlach ^[1] ; Vincent,Rioux ^[4]

作者单位： Institut Agro, INSERM, INRAE, Univ Rennes, NuMeCan, Rennes 35000, France; INSERM, INRAE, Univ Rennes, NuMeCan, Rennes 35000, France. ^[1] Institut Agro, INSERM, INRAE, Univ Rennes, NuMeCan, Rennes 35000, France. ^[2] INSERM, INRAE, Univ Rennes, NuMeCan, Rennes 35000, France. ^[3] Institut Agro, INSERM, INRAE, Univ Rennes, NuMeCan, Rennes 35000, France; INSERM, INRAE, Univ Rennes, NuMeCan, Rennes 35000, France. Electronic address: vincent.rioux@institut-agro.fr. ^[4]

关键词 Fatty acid desaturases In vitro activity measurement Kinetic parameters Liver Negative chemical ionization mass spectrometry Non-radioactive analytical method PFB esters