Systematic comparison of quantity and quality of RNA recovered with commercial FFPE tissue extraction kits.

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作者： Sukoluhle,Dube ^[1] ; Sharefa,Al-Mannai ^[2] ; Li,Liu ^[3] ; Sara,Tomei ^[3] ; Satanay,Hubrack ^[4] ; Shimaa,Sherif ^[2] ; Ayesha,Jabeen ^[2] ; Eiman I,Ahmed ^[5] ; Apryl,Sanchez ^[2] ; William,Mifsud ^[6] ; Davide,Bedognetti ^[2] ; Wouter,Hendrickx ^[7] ; Christophe M,Raynaud ^[8]

作者单位： Anatomical Pathology Lab, Department of Pathology, Sidra Medicine, Doha, Qatar. ^[1] Tumour Biology and Immunology Laboratory, Research Branch, Sidra Medicine, Doha, Qatar. ^[2] Integrated Genomic Services, Sidra Medicine, Doha, Qatar. ^[3] Laboratory of Immunoregulation, Research Branch, Sidra Medicine, Doha, Qatar. ^[4] Tumour Biology and Immunology Laboratory, Research Branch, Sidra Medicine, Doha, Qatar.;Department of Biomedical Science, College of Health Sciences, Qatar University, Doha, Qatar. ^[5] Anatomical Pathology Lab, Department of Pathology, Sidra Medicine, Doha, Qatar.;Tumour Biology and Immunology Laboratory, Research Branch, Sidra Medicine, Doha, Qatar. ^[6] Tumour Biology and Immunology Laboratory, Research Branch, Sidra Medicine, Doha, Qatar.;College of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Doha, Qatar. ^[7] Tumour Biology and Immunology Laboratory, Research Branch, Sidra Medicine, Doha, Qatar. craynaud@sidra.org. ^[8]

关键词 FFPE RNA extraction kit RNA quality RNA quantity

主题词石蜡包埋(Paraffin Embedding);人类(Humans);RNA(RNA);组织固定(Tissue Fixation);试剂盒, 诊断(Reagent Kits, Diagnostic);甲醛(Formaldehyde)

DOI 10.1186/s12967-024-05890-5

PMID 39762904

发布时间 2025-01-06

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