Engineering high-activity crosslinked enzyme aggregates via SpyCatcher/SpyTag-mediated self-assembly.

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第一作者： Xueliang,Ma

第一单位： College of Forestry, Northwest A&F University, No.3 Taicheng Road, Yangling, Shaanxi 712100, China.

作者： Xueliang,Ma ^[1] ; Yu,Yang ^[1] ; Manzhu,Wang ^[1] ; Yubo,Zhang ^[1] ; Bingxuan,Zhao ^[1] ; Shibo,Hao ^[1] ; Lili,Jia ^[2]

作者单位： College of Forestry, Northwest A&F University, No.3 Taicheng Road, Yangling, Shaanxi 712100, China. ^[1] College of Forestry, Northwest A&F University, No.3 Taicheng Road, Yangling, Shaanxi 712100, China; Shaanxi Key Laboratory of Economic Plant Resources Development and Utilization, Yangling, Shaanxi 712100, China. Electronic address: lilijia@nwsuaf.edu.cn. ^[2]

关键词 Cellulase Crosslinked enzyme aggregates Multienzyme complex Self-assembly SpyCatcher/SpyTag

主题词酶类, 固相(Enzymes, Immobilized);蛋白质工程(Protein Engineering);交联试剂(Cross-Linking Reagents);酶稳定性(Enzyme Stability);纤维素酶(Cellulase);戊二醛(Glutaral);内-1,4-β-木聚糖酶类(Endo-1,4-beta Xylanases)

DOI 10.1016/j.ijbiomac.2025.139591

PMID 39788221

发布时间 2025-02-19

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