Bifunctional probe propelling multipath strand displacement amplification tandem CRISPR/Cas12a for ultrasensitive and robust assay of DNA methyltransferase activity.

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第一作者： Fei,Yu

第一单位： College of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, China.

作者： Fei,Yu ^[1] ; Qiongwen,Zhang ^[1] ; Tiantian,Ma ^[2] ; Shuying,Zhang ^[1] ; Fanting,Wang ^[1] ; Dan,Yue ^[1] ; Shihan,Liu ^[1] ; Yueqi,Liao ^[1] ; Li-E,Liu ^[1] ; Yongjun,Wu ^[3] ; Wenqiao,Zang ^[4]

作者单位： College of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, China. ^[1] College of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, China; General Hospital of Xuzhou Mining Group, Xuzhou, Jiangsu, 221006, China. ^[2] College of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, China. Electronic address: wuyongjun@zzu.edu.cn. ^[3] School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China. Electronic address: zangwenqiao@zzu.edu.cn. ^[4]

关键词 Bifunctional hairpin probe DNA methyltransferase activity assay Multipath strand displacement amplification Tandem CRISPR/Cas12a

主题词核酸扩增技术(Nucleic Acid Amplification Techniques);DNA甲基化(DNA Methylation);检测限(Limit of Detection);DNA修饰甲基酶类(DNA Modification Methylases);酶测定(Enzyme Assays);DNA探针(DNA Probes)

DOI 10.1016/j.aca.2024.343540

PMID 39800499

发布时间 2025-01-12

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