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Development of an efficient expression system for human chaperone BiP in Pichia pastoris: production optimization and functional validation.

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第一作者： Eimantas,Žitkus

第一单位： Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius, LT-10257, Lithuania.;Department of Eukaryote Gene Engineering, Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius, LT-10257, Lithuania.

作者： Eimantas,Žitkus ^[1] ; Evaldas,Čiplys ^[2] ; Mantas,Žiaunys ^[3] ; Andrius,Sakalauskas ^[3] ; Rimantas,Slibinskas ^[4]

作者单位： Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius, LT-10257, Lithuania.;Department of Eukaryote Gene Engineering, Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius, LT-10257, Lithuania. ^[1] Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius, LT-10257, Lithuania. evaldas.ciplys@bti.vu.lt.;Department of Eukaryote Gene Engineering, Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius, LT-10257, Lithuania. evaldas.ciplys@bti.vu.lt. ^[2] Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius, LT-10257, Lithuania. ^[3] Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius, LT-10257, Lithuania. rimantas.slibinskas@bti.vu.lt.;Department of Eukaryote Gene Engineering, Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius, LT-10257, Lithuania. rimantas.slibinskas@bti.vu.lt. ^[4]

关键词 Pichia pastoris BiP DTT Fermentation Mineral medium Mixed feeding Secretion

医学主题词人类(Humans);重组蛋白质类(Recombinant Proteins);发酵(Fermentation);酵母菌目(Saccharomycetales);热休克蛋白质类(Heat-Shock Proteins);培养基(Culture Media);毕赤酵母(Pichia)

DOI 10.1186/s12934-025-02679-z

PMID 40102863

发布时间 2025-05-14

基金项目

01.2.2-LMT-K-718-03-0021/European Regional Development Fund

翻译满意度评价：

准确一般字义偏差语意偏离译文作用小提交