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Enhancing catalytic activity of thermostable 4-α-glucanotransferase from Thermus filiformis through semi-rational design.

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第一作者： Qian,Wang

第一单位： Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

作者： Qian,Wang ^[1] ; Jiao,Li ^[2] ; Heyue,Li ^[1] ; Jingyu,Zhang ^[2] ; Erbing,Hua ^[3] ; Huimin,Qin ^[3] ; Peng,Chen ^[4] ; Yuanxia,Sun ^[5]

作者单位： Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. ^[1] Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. ^[2] Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. ^[3] Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. Electronic address: chen_p@tib.cas.cn. ^[4] Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. Electronic address: sun_yx@tib.cas.cn. ^[5]

关键词 4-α-glucanotransferase Catalytic activity Semi-rational design Site-saturation mutation Substrate channel