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Functional characterization and directed evolution of Cicer arietinum glutathione transferases for enhanced hydroperoxidase activity and ligandin function.

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第一作者： Anni,Kontouri

第一单位： Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece.

作者： Anni,Kontouri ^[1] ; Nikolaos,Georgakis ^[1] ; Anastassios C,Papageorgiou ^[2] ; Nikolaos Ε,Labrou ^[3]

作者单位： Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece. ^[1] Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, 20521, Finland. ^[2] Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece. Electronic address: Lambrou@aua.gr. ^[3]

关键词 Cicer arietinum DNA shuffling Directed evolution Fabaceae Glutathione transferases Hydroperoxidase activity Pesticide binding Xenobiotic detoxification

医学主题词谷胱甘肽转移酶(Glutathione Transferase);鹰觜豆(Cicer);定向分子进化(Directed Molecular Evolution);动力学(Kinetics);氨基酸序列(Amino Acid Sequence);二硝基氯苯(Dinitrochlorobenzene);苯衍生物(Benzene Derivatives)

DOI 10.1016/j.phytochem.2025.114692

PMID 41022292

发布时间 2025-12-06

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