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Expression, purification, and biophysical characterization of liquid-liquid phase separation of full-length hnRNPA2B1.

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第一作者： Luis Fernando,Durán-Armenta

第一单位： VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie, 1050, Brussels, Belgium; Structural Biology Brussels, Department of Bioengineering Sciences, Vrije Universiteit Brussel, 1050, Brussels, Belgium.

作者： Luis Fernando,Durán-Armenta ^[1] ; Attila,Meszaros ^[1] ; Julia,Malo Pueyo ^[2] ; Steven,Janvier ^[1] ; Tamas,Lazar ^[1] ; Dominique,Maes ^[3] ; Remy,Loris ^[1] ; Peter,Tompa ^[4]

作者单位： VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie, 1050, Brussels, Belgium; Structural Biology Brussels, Department of Bioengineering Sciences, Vrije Universiteit Brussel, 1050, Brussels, Belgium. ^[1] VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie, 1050, Brussels, Belgium; Structural Biology Brussels, Department of Bioengineering Sciences, Vrije Universiteit Brussel, 1050, Brussels, Belgium; Brussels Center for Redox Biology, Vrije Universiteit Brussel, 1050, Brussels, Belgium. ^[2] Structural Biology Brussels, Department of Bioengineering Sciences, Vrije Universiteit Brussel, 1050, Brussels, Belgium. ^[3] VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie, 1050, Brussels, Belgium; Structural Biology Brussels, Department of Bioengineering Sciences, Vrije Universiteit Brussel, 1050, Brussels, Belgium; Institute of Molecular Life Sciences, HUN-REN Research Centre for Natural Sciences, 1117, Budapest, Hungary; HUN-REN Office for Supported Research Groups (TKI), Cell Cycle Laboratory, National Institute of Oncology, 1122, Budapest, Hungary. Electronic address: peter.tompa@vub.be. ^[4]

关键词 Condensate maturation Intrinsically disordered proteins Liquid-liquid phase separation Low-complexity domain RNA-Binding protein hnRNPA2B1

医学主题词核不均一核糖核蛋白A-B组(Heterogeneous-Nuclear Ribonucleoprotein Group A-B);人类(Humans);重组蛋白质类(Recombinant Proteins);基因表达(Gene Expression);大肠杆菌(Escherichia coli)

DOI 10.1016/j.pep.2025.106861

PMID 41297573

发布时间 2026-01-16

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