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RT-LAMP-CRISPR/Cas12b-based hand-pressure-actuated microfluidic chip for rapid and portable detection of severe fever with thrombocytopenia syndrome virus.

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第一作者： Yan,Long

第一单位： School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China. Electronic address: longy73@mail2.sysu.edu.cn.

作者： Yan,Long ^[1] ; Shuting,Sun ^[2] ; Haokun,Mei ^[3] ; Deqian,Zhou ^[4] ; Huanning,Zhou ^[5] ; Zhaoyang,Fang ^[6] ; Xiaokang,Li ^[7] ; Ning,Li ^[8] ; Tianchi,Zhuang ^[9] ; Cheng,Guo ^[10]

作者单位： School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China. Electronic address: longy73@mail2.sysu.edu.cn. ^[1] The Second School of Clinical Medicine, Nanjing Medical University, Nanjing, 210006, China. Electronic address: 13535380519@163.com. ^[2] The Forth School of Clinical Medicine, Nanjing Medical University, Nanjing, 210006, China. Electronic address: mei1150187542@163.com. ^[3] Yuexiu District Center for Disease Control and Prevention, Guangzhou, 511430, China. Electronic address: 21497066@qq.com. ^[4] Yuexiu District Center for Disease Control and Prevention, Guangzhou, 511430, China. Electronic address: 80456861@qq.com. ^[5] School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China. Electronic address: fangzhaoyang02@163.com. ^[6] School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China. Electronic address: lixk35@mail2.sysu.edu.cn. ^[7] Jiangning District Center for Disease Control and Prevention, Jiangsu, 211199, China. Electronic address: liningreal@163.com. ^[8] School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China. Electronic address: 945998089@qq.com. ^[9] School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China; National Medical Products Administration Key Laboratory for Quality Monitoring and Evaluation of Vaccines and Biological Products, Guangzhou, 510080, China. Electronic address: guoch36@mail.sysu.edu.cn. ^[10]

关键词 CRISPR/Cas12b Microfluidic chip POC testing RT-LAMP Severe fever with thrombocytopenia syndrome virus

医学主题词白蛉病毒(Phlebovirus);核酸扩增技术(Nucleic Acid Amplification Techniques);人类(Humans);芯片实验室装置(Lab-On-A-Chip Devices);分子诊断技术(Molecular Diagnostic Techniques);压力(Pressure);RNA, 病毒(RNA, Viral)

DOI 10.1016/j.talanta.2025.129277

PMID 41448060

发布时间 2026-01-15

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