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Offsetting ROS-Mediated Arrest of Endothelial Fenestration Dynamics Permits Long-Term Optical Super-Resolution Imaging Validated by AFM.

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第一作者： Annika,Kiel

第一单位： Department of General- and Visceral Surgery - Liver- and Tumor Biology, Medical Faculty OWL, Bielefeld University, Bielefeld 33615, Germany.

作者： Annika,Kiel ^[1] ; Marcin,Luty ^[2] ; Angela,Kralemann-Köhler ^[1] ; Laureen Patricia,Helweg ^[1] ; Jasmin,Schürstedt-Seher ^[3] ; Jerzy,Kotlinowski ^[4] ; Jakub,Pospíšil ^[5] ; Malgorzata,Lekka ^[6] ; Thanh-Diep,Ly ^[1] ; Thomas,Huser ^[3] ; Jan,Schulte Am Esch ^[7] ; Wolfgang,Hübner ^[3] ; Karolina,Szafranska ^[5] ; Bartlomiej,Zapotoczny ^[6]

作者单位： Department of General- and Visceral Surgery - Liver- and Tumor Biology, Medical Faculty OWL, Bielefeld University, Bielefeld 33615, Germany. ^[1] Department of Biophysical Microstructures, Institute of Nuclear Physics, Polish Academy of Sciences, Ul. Radzikowskiego 142, Krakow 31-342, Poland.;Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, Krakow 31-034, Poland. ^[2] Biomolecular Photonics, Faculty of Physics, Bielefeld University, Bielefeld 33615, Germany. ^[3] Department of General Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow 30-387, Poland. ^[4] Vascular Biology Research Group, Department of Medical Biology, University of Tromsø─The Arctic University of Norway, Tromsø 9019, Norway. ^[5] Department of Biophysical Microstructures, Institute of Nuclear Physics, Polish Academy of Sciences, Ul. Radzikowskiego 142, Krakow 31-342, Poland. ^[6] Department of General- and Visceral Surgery - Liver- and Tumor Biology, Medical Faculty OWL, Bielefeld University, Bielefeld 33615, Germany.;Department of General and Visceral Surgery, University Hospital OWL of the University of Bielefeld, Campus Bielefeld-Bethel, Bielefeld 33501, Germany. ^[7]

关键词 atomic force microscopy (AFM)fenestrations live-cell imaging liver sinusoidal endothelial cells (LSEC)reactive oxygen species (ROS)structured illumination microscopy (SIM)super-resolution microscopy

医学主题词活性氧(Reactive Oxygen Species);内皮细胞(Endothelial Cells);显微镜检查, 原子力(Microscopy, Atomic Force);动物(Animals);显微镜检查, 荧光(Microscopy, Fluorescence);肝(Liver);人类(Humans)

DOI 10.1021/acsami.5c22333

PMID 41486682

发布时间 2026-01-15

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