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Novel nucleic acid detection based on the guide-independent nuclease activity of TbAgo.

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第一作者： Mengjun,Fang

第一单位： Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China; Children's Health Innovation Research Center, Binjiang Institute of Zhejiang University, Hangzhou, China.

作者： Mengjun,Fang ^[1] ; Di,Huang ^[2] ; Peijie,Shen ^[3] ; Mingyan,Li ^[3] ; Wei,Li ^[4] ; Chunzhen,Hua ^[5] ; Zehua,Bao ^[6] ; Qiang,Shu ^[7] ; Zhinan,Xu ^[8]

作者单位： Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China; Children's Health Innovation Research Center, Binjiang Institute of Zhejiang University, Hangzhou, China. ^[1] National Clinical Research Center for Child Health, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ^[2] Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China. ^[3] Department of Clinical Laboratory, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ^[4] Department of Infectious Diseases, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ^[5] Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China. Electronic address: zbao@zju.edu.cn. ^[6] Children's Health Innovation Research Center, Binjiang Institute of Zhejiang University, Hangzhou, China. Electronic address: shuqiang@zju.edu.cn. ^[7] Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China; Hangzhou FasTech Biotechnology Co., Ltd, Hangzhou, China. Electronic address: znxu@zju.edu.cn. ^[8]

关键词 Chopping activity Multiplexed nucleic acid detection One-pot detection Prokaryotic Argonaute