Three of four cysteines, including that responsible for substrate activation, are ionized at pH 6.0 in yeast pyruvate decarboxylase: evidence from Fourier transform infrared and isoelectric focusing studies.
第一作者:
I,Baburina
第一单位:
Department of Chemistry, Rutgers, State University of New Jersey, Newark 07102, USA.
作者:
医学主题词
半胱氨酸(Cysteine);DNA引物(DNA Primers);酶激活(Enzyme Activation);氢离子浓度(Hydrogen-Ion Concentration);等电聚焦(Isoelectric Focusing);诱变, 定点(Mutagenesis, Site-Directed);丙酮酸脱羧酶(Pyruvate Decarboxylase);酿酒酵母菌(Saccharomyces cerevisiae);谱学, 傅里叶变换红外(Spectroscopy, Fourier Transform Infrared);底物特异性(Substrate Specificity)
DOI
10.1021/bi961141+
PMID
8756679
发布时间
2013-11-21
- 浏览18
Biochemistry
10249-55页
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