Improving maltodextrin specificity for enzymatic synthesis of 2-O-D-glucopyranosyl-L-ascorbic acid by site-saturation engineering of subsite-3 in cyclodextrin glycosyltransferase from Paenibacillus macerans

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作者： Liu, Long ^[1] ; Xu, Qiaoyan ^[2] ; Han, Ruizhi ^[3] ; Shin, Hyun-dong ^[4] ; Chen, Rachel R. ; Li, Jianghua ; Du, Guocheng ; Chen, Jian

作者单位： National Engineering of Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China ^[1] School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta 30332, USA ^[2] Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China ^[3] Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China ^[4]

关键词 Cyclodextrin glycosyltransferase (CGTase)l-Ascorbic acid (l-AA)Maltodextrin 2-O-Glucopyranosyl-l-ascorbic acid (AA-2G)Site-saturation mutagenesis

发布时间 2013-11-20

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