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The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China

摘要Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qjang Autonomous Prefecture in the Sichuan province of China.Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically (following modified acid fast staining). In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed.Results The prevalence of Cryptosporidium in the collected samples was at 14.6% (31/213) and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype (Cryptosporidium ubiquitum) demonstrating that this species was the primary sheep species found in sheep in China.Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.

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分类号 S858.2
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DOI 10.3967/0895-3988.2011.03.016
发布时间 2011-09-20(万方平台首次上网日期,不代表论文的发表时间)
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生物医学与环境科学(英文版)

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