摘要目的 探讨小鼠红白血病细胞FBL-3的生物学特性.方法 观察小鼠红白血病FBL-3细胞光镜下的形态、生长曲线、体外克隆形成率及细胞免疫组织化学染色情况;流式细胞术分析细胞周期分布以及MHC分子的表达情况;常规细胞遗传学方法分析染色体核型,PCR检测性别基因Sry;MTT法分析化疗药物敏感性;经尾静脉接种后观察C57BL/6小鼠肝、脾、肺、肾的病理变化.结果 FBL-3细胞呈梭形或多角形贴壁生长;细胞群体倍增时间为24.12 h.14 d和21 d克隆形成率分别为(35.23±1.44)%和(60.27±5.56)%.糖原染色阳性、氯醋酸染色部分阳性,过氧化物酶、碱性磷酸酶、丁酸染色均阴性.细胞周期:G0/G1细胞占(50.9±2.5)%,S期细胞占(36.3±1.4)%、G2/M期细胞占(13.8±0.8)%.对化疗药物阿糖胞苷、长春地辛、顺铂、丝裂霉素、甲氨蝶呤的半数抑制浓度分别为(0.49±0.04)、(0.87±0.09)、(3.77±0.32)、(1.66±0.16)μmol/L和(2.77±0.24)nmol/L.FBL-3细胞染色体数目在34～41条之间,表达H-2b分子,Sry基因阳性.静脉接种小鼠100%发病;接种的细胞数量与存活时间呈线性关系;白血病细胞可以浸润肝、肺、脾和肾.结论 FBL-3细胞具有白血病细胞的特性,体外易于培养,容易建立小鼠模型,可作为研究白血病的理想动物模型.

abstractsObjective To investigate the biological characteristics of mouse erythrolenkemia cell FBL-3.Methods The morphological feature, growth characters, clone formation and immunochemistry of mouse erythroleukemia cell FBL-3 were examined by light microscope. The cell cycle distribution and expression of MHC were detected by flow cytometry. Drug sensitivity was measured by MTT assay. Tumorigenicity was evaluated after intravenous injection FBL-3 cells into C57BL/6 mice. Results FBL-3 cells were smaller,fusiform or polygon, adherence. Doubling time was 24.12 h. The clone formation rates were (35.23±1.44) %and (60.27±5.56) % at 14 th and 21 th day, respectively. The reactions for PAS and chloracetic acid were positive, while the POX, NAP and butanoic acid reactions were negative. The cell cycle distribution was as follow: G0/G1 phase (50.9±2.5) %, S phase (36.3±1.4) %, G2/M phase (13.8±0.8) %. The IC50 of FBL-3 cells to Ara-C, VDS, DDP, MMC and MTX were (0.49±0.04), (0.87±0. 09), (3.77±0.32), (1.66±0.16) μmol/L and (2.77±0.24) nmol/L, respectively. Chromosome number was at 34 to 41. MHC of FBL-3 cell was H-2b. Sexual gene Sry was positive. All C57BL/6 mice were morbidity with erythroblastic leukemia when FBL-3 cells had been intravenously inoculated. There was a linear relationship between the survival time and the number cell injected. The main targets for the leukemic FBL-3 cells were liver, spleen, kindey and lung. Conclusion FBL-3 cell has typical features of mouse leukemia cell, was easily cultured in vitro and tumorigenesised in C57BL/6 mice. FBL-3 cell could be as a satisfactory tool for the research of leukemia.