摘要目的 探讨人类急性白血病(AL)细胞中肿瘤抑制基因1(TIG1)的表达及其与预后的关系.方法 应用实时荧光定量聚合酶链反应( RT-QT-PCR)的方法,检测75例AL患者的TIG1表达情况,并进一步观察其与缓解率的关系.20名健康人作对照.结果 TIG1在健康对照组中的表达水平高( 0.0609±0.0281),而在初治AL中表达水平明显减低(0.0057±0.0035),两组比较差异有统计学意义(U=-6.321,P=0.000)；缓解组患者表达水平(0.0409±0.0244)也较健康对照组减低(U=-2.582,P=0.01),且缓解组患者的表达水平也明显高于初治白血病组(U=-6.366,P=0.000)；TIG1高表达患者的缓解率为83.3％(15/18)明显高于低表达患者的53.1％( 17/32)(x2=4.563,P=0.033).结论 TIG1基因表达的减少与AL的发病有关,而且低表达患者的预后不佳.

abstractsObjective To investigate correlation between the expression of TIG1 and the prognostic significance in acute leukemia (AL).Methods The TIG1 expression level of 75 AL and 20 normal control (NC) were measured by Real-Time Quantitative PCR(RT-QT-PCR).Results TIG1 gene expressed at a higher level in NC(0.0609±0.0281 ) than that in de novo AL(0.0057±0.0035)(U=-6.321,P=0.000).The expression of TIG1 in complete remission patients(AL-CR)(0.0409±0.0244)were lower than that in NC too(U=-2.582,P=0.01).The expression of TIG1 in AL-CR patients (83.3 ％) were higher than that in de novo AL (53.1％)(U=-6.366,P=0.000).In AL patients,the CR rate of high TIG1 level cases were higher than that in low level cases (x2=4.563,P=0.033).Conclusions Reduced expression of TIG1 may play an important role in the pathogenesis of AL.TIG1 can serve as a marker of poor prognosis in AL.