摘要Background:To explore the effect of electroacupuncture Wushu acupoints on angiogenesis and expressions of brain-derived neurotrophic factor(BDNF),as well as to explore the possible mechanism of electroacupuncture on acute myocardial ischemia(AMI).Methods:We randomly divided 42 Sprague Dawley rats into sham AMI group,AMI group,and the heart channel groups comprising Shaochong(HT9)acupoint,Shaofu(HT8)acupoint,Shenmen(HT7)acupoint,Lingdao(HT4)acupoint,and Shaohai(HT3)acupoint groups,with 6 rats in each group.The AMI model was fabricated via hypodesmus of the left anterior descending coronary artery.After modeling,the corresponding acupoints of each group underwent electroacupuncture treatment 30 min/time,once/day for 3 consecutive days.The cardiogram obtained before and after the intervention was compared,and the pathologic changes of the myocardial tissue were observed via hematoxylin&eosin staining.The amount of serum endothelin-1,prostacycline-2,thromboxane-2,and BDNF indicators were assayed using ELISA,the number of CD31-positive cells in myocardial tissue was calculated using immunohistochemistry,and the expression levels of BDNF and TrKB was measured using western blot.Result:Compared with the sham AMI group,the ST segment on the electrocardiogram was significantly elevated(P<0.05);the myocardial angiogenesis marker CD31 was remarkably increased(P<0.05);serum levels of endothelin-1,prostacycline-2 and prostacycline-2/thromboxane-2 were decreased(P<0.05),whereas serum levels of thromboxane-2 levels were increased(P<0.05);and those of serum BDNF was decreased(P<0.05);the protein overexpression contents of BDNF and TrKB in the myocardial tissues of HT8 acpoint and Shenmen acpoint groups were reduced(P<0.05)in the AMI group.Compared with the AMI group,in the heart channel groups,the ST segment of electrocardiogram was dramatically reduced(P<0.05);CD31 was dramatically elevated(P<0.05);the serum levels of endothelin-1,prostacycline-2,and prostacycline-2/thromboxane-2 were increased(P<0.05),thromboxane-2 levels were reduced(P<0.05);the serum BDNF level was increased(P<0.05);the protein overexpression levels of BDNF and TrKB in myocardial tissue of HT8 group and Shenmen group was increased(P<0.05).Conclusion:electroacupuncture of Wushu acupoints along the heart channel may enhance myocardial ischemia by promoting overexpression of serum BDNF and activating the BDNF/TrKB pathway to promote myocardial angiogenesis.