摘要Objective:To detect the expression of RECK gene in the highly and low metastatic cell sublines of human os-teosarcema cell HOS and explore its possible roles on the occurrence and metastasis of osteosarcoma. Methods:RT-PCR, gelatin zymography, and rnatrigel invasion assay were respectively used to evaluate the endogenous expression of RECK mRNA, MMP-2 activation ratio and invesive capacity in the two osteosarcoma cell sublines. Results:The highly metastatic cell group expressed significantly lower mRNA level of RECK than the low metastatic cell group (P < 0.05), but showed higher MMP-2 activation ratio and invasive capacity (P < 0.05 and P < 0.01, respectively). Conclusion:The abnormal low expression of RECK may participate in osteosarcoma invasion and metastasis, and may be a new therapeutic target for osteosarooma.