摘要Background:Human papillomavirus(HPV)infection is a major risk factor of cervical cancer.This study assessed the prevalence and distribution of HPV genotypes in women with cervical or vaginal lesions in Jiangsu Province,China.Methods:A total of 2120 healthy women aged 18-45 years were screened between 2012 and 2013 and 6171 healthy women aged 18-45 years were screened between 2020 and 2021 in Jiangsu Province.Cervical specimens collected from each woman were first tested using the HPV DNA enzyme immunoassay method,and positive samples were further tested using the reverse hybridization line probe assay.Differences in HPV prevalence and genotype distribution were compared between women with cervical and vaginal lesions identified during 2 rounds of cross-sectional screening.To account for differences in age composition between the 2 studies,the stan-dardized prevalence of HPV positivity was calculated using the sum of the total number of women diagnosed with cervical or vaginal le-sions during both periods as the standard group.Results:Overall,40 women(1.89％)were diagnosed with cervical or vaginal lesions through biopsy during 2012-2013,and 110(1.78％)were diagnosed during 2020-2021.Among women with lesions,the standardized HPV positivity rates were 98.41％in 2012-2013 and 99.24％in 2020-2021.Most cases were caused by high-risk HPV,which accounted for 87.18％of the total infections during 2012-2013 and 89.91％of those in 2020-2021,with standardized positivity rates of 86.44％and 88.75％,respectively.The standardized positivity rates for single infections were 62.35％in 2012-2013 and 74.95％in 2020-2021.The top 5 high-risk HPV genotypes were HPV type 16(HPV16;29.01％),52(20.63％),18(14.28％),58(13.71％),and 33(12.12％)in 2012-2013,and HPV16(36.95％),58(22.18％),52(13.25％),31(7.63％),and 51(6.81％)in 2020-2021.The standardized positivity rate for HPV18 decreased from 14.28％in 2012-2013 to 1.15％in 2020-2021.Among women with cervical or vaginal lesions,the highest proportion was observed in the 36-to 45-year group during 2012-2013,reaching 52.50％,and in the 26-to 35-year group during 2020-2021,peaking at 59.10％.Conclusions:In Jiangsu Province,no significant changes in HPV prevalence among women with cervical or vaginal lesions were ob-served during 2012-2013 and 2020-2021;however,the distribution of HPV genotypes had changed.