摘要Background:Chronic obstructive pulmonary disease(COPD)is a type of chronic res-piratory disease.Studies confirmed that ferroptosis was involved in the progression of COPD,and its related mechanism is not clear.The aim of this study was to identify ferroptosis-related genes and reveal its pathological application in COPD.Methods:First,we downloaded two datasets from the Gene Expression Omnibus(GEO)to obtain the differential genes of COPD.Ferroptosis-related genes were ob-tained from the ferroptosis database,FerrDb.Next,we obtained the key genes in COPD rat to identify potential biomarkers using quantitative real-time polymerase chain reaction.Ferroptosis and inflammation were assessed using hematoxylin and eosin staining,lung function tests,and transmission electron microscopy(TEM).Results:These results were used to construct a COPD risk model with six key genes and explore the immunological characteristics of these genes.The resulting mo-lecular subtype construction confirmed the importance of the key genes in COPD.Furthermore,we proved that ferroptosis occurred in the COPD rat model and identi-fied the six key genes in rat lung tissue.TEM showed significant functional impairment and structural alterations in mitochondria,which is the key site of ferroptosis.Conclusion:Our COPD risk model,incorporating six key genes,highlighted their im-munological roles in COPD using bioinformatics analysis and in vivo experiments.We hope to provide the basis for the treatment targeting ferroptosis in COPD.