摘要The small-island effect (SIE) has become more and more part of the theoretical framework of island biogeography and biodiversity research.However,previous methods for the detection of SIEs are often flawed in one way or another,including not accounting for model complexity,not comparing all relevant models,and not including islands with no species.Therefore,the existence and the prevalence of the SIE may be dubious.In this study,after controlling for all these methodological shortcomings,we tested for the existence of the SIE in amphibian assemblages on subtropical landbridge islands created by the inundation of the Thousand Island Lake,China.We used the line transect method to determine the distribution of amphibian assemblages on 23 study islands during 3breeding seasons from 2009 to 2011.To evaluate whether an SIE exists in amphibian assemblages,we compared the fit of a simple linearized power model with two most widely used breakpoint regression models.The information-theoretic multimodel inference approach based on Akaike's information criterion identified the left-horizontal SIE model as the best single model.Thus,we found strong evidence for the existence of an SIE in our system.The upper limit of the SIE for amphibian assemblages was 39.95 ha.Below this threshold area,amphibian richness varied independently of island size.The SIE in amphibian assemblages may be due to episodic disturbances,stochastic events,and nutrient subsidies from the lake.Our results indicate that all the islands ＞39.95 ha should be protected for the effective conservation of amphibian assemblages in our system.