摘要Prokaryotic type Ⅱ adaptive immune systems have been developed into the versatile CRISPR technology,which has been widely applied in sitespecific genome editing and has revolutionized biomedical research due to its superior efficiency and flexibility.Recent studies have greatly diversified CRISPR technologies by coupling it with various DNA repair mechanisms and targeting strategies.These new advances have significantly expanded the generation of genetically modified animal models,either by including species in which targeted genetic modification could rot be achieved previously,or through introducing complex genetic modifications that take multiple steps and cost years to achieve using traditional methods.Herein,we review the recent developments and applications of CRISPR-based technology in generating various animal models,and discuss the everlasting impact of this new progress on biomedical research.