摘要The abundance of domesticated sheep varieties and phenotypes is largely the result of long-term natural and artificial selection.However,there is limited information regarding the genetic mechanisms underlying phenotypic variation induced by the domestication and improvement of sheep.In this study,to explore genomic diversity and selective regions at the genome level,we sequenced the genomes of 100 sheep across 10 breeds and combined these results with publicly available genomic data from 225 individuals,including improved breeds,Chinese indigenous breeds,African indigenous breeds,and their Asian mouflon ancestor.Based on population structure,the domesticated sheep formed a monophyletic group,while the Chinese indigenous sheep showed a clear geographical distribution trend.Comparative genomic analysis of domestication identified several selective signatures,including IFI44 and IFI44L genes and PANK2 and RNF24 genes,associated with immune response and visual function.Population genomic analysis of improvement demonstrated that candidate genes of selected regions were mainly associated with pigmentation,energy metabolism,and growth development.Furthermore,the IFI44 and IFI44L genes showed a common selection signature in the genomes of 30 domesticated sheep breeds.The IFI44 c.54413058 C＞G mutation was selected for genotyping and population genetic validation.Results showed that the IFI44 polymorphism was significantly associated with partial immune traits.Our findings identified the population genetic basis of domesticated sheep at the whole-genome level,providing theoretical insights into the molecular mechanism underlying breed characteristics and phenotypic changes during sheep domestication and improvement.