摘要The objective of this study was to investigate whether supplementation with N-carbamoylglutamate (NCG)to cows during late gestation alters uteroplacental tissue nutrient transporters,calf metabolism and newborn weight.Thirty multiparous Chinese Holstein cows were used in a randomized complete block design experiment.During the last 28 d of pregnancy,cows were fed a diet without (CON) or with NCG (20 g/d per cow).The body weight of calves was weighed immediately after birth.Placentome samples were collected at parturition and used to assess mRNA expression of genes involved in transport of arginine,glucose,fatty acid and angiogenesis factors,as well as the mammalian target of rapamycin (mTOR) pathway.Blood samples of calves before colostrum consumption were also collected for the detection of plasma parameters,amino acids (AA) and metabolomics analysis.The newborn weight (P =0.02) and plasma Arg concentration of NCG-calves was significantly higher (P =0.05) than that of CON-calves,and the plasma concentrations of urea nitrogen tended to be lower (P =0.10) in the NCG group.The mRNA abundance of genes involved in glucose transport (solute carrier family 2 member 3[SLC2A3],P ＜ 0.01),angiogenesis(nitric oxide synthase 3[NOS3],P =0.02),and mTOR pathway (serine/threonine-protein kinase 1[AKT1],P =0.10;eukaryotic translation initiation factor4B pseudogene 1[EIF4BP1],P =0.08;EIF4EBP2,P =0.04;and E74-like factor 2[ELF2],P =0.03) was upregulated in the placentome of NCG-supplemented cows.In addition,17 metabolites were significantly different in the placentome of NCG-supplemented cows compared to non-supplemented cows,and these metabolites are mainly involved in arginine and proline metabolism,alanine,aspartate and glutamate metabolism,and citrate cycle.In summary,the increased body weight of newborn calves from the NCG supplemented dairy cows may be attributed to the increased angiogenesis and uteroplacental nutrient transport and to the activated mTOR signal pathway,which may result in the increased nutrient supply to the fetus,and improved AA metabolism and urea cycle of the fetus.