摘要Phosphorus metabolism in laying hens is a highly dynamic process over the course of the 24 h egg-laying cycle.Adjusting the phosphorus feeding regimen according to the daily egg-laying cycle may help to improve phosphorus utilization efficiency.Hy-Line Brown layers(n=120;70 wk old)were offered 4 different phosphorus daily regimens:(1)RR,fed regular phosphorus at both 09:00 and 17:00;(2)RL,fed regular phosphorus at 09:00 and low phosphorus at 17:00;(3)LR,fed low phosphorus at 09:00 and regular phosphorus at 17:00;(4)LL,fed low phosphorus at both 09:00 and 17:00.The regular and low phosphorus diets contained 0.32％and 0.14％non-phytate phosphorus,respectively.The feeding trial lasted for 12 wk.As a result,layers on the RL regimen had decreased laying rate(P＜0.05;5 to 8,9 to 12,and 1 to 12 wk)when compared to all other regimens.Layers on the LL regimen had decreased eggshell thickness and specific gravity(P＜0.05;wk 8)when compared to all other regimens,and had decreased egg shell strength(P＜0.05;wk 8)when compared to RL and LR regimens.When compared to the RR regimen(a common practice in the industry),layers on the LR regimen had:(1)identical laying per-formance and egg quality(P＞0.05);(2)decreased phosphorus excretion(P＜0.05)during the period of 09:00 to 17:00;(3)increased jejunal calbindin D28k protein expression(P＜0.05)2 h after feeding in the morning;(4)decreased serum fibroblast growth factor 23 and calcitriol levels(P＜0.05),decreased jejunal type Ⅲ sodium-phosphate cotransporter 2 gene and protein expression(P＜0.05),and decreased renal type Ⅲ sodium-phosphate cotransporter 1 protein expression(P＜0.05),2 h after feeding in the afternoon.In summary,when dietary phosphorus was supplemented in accordance with daily serum phosphorus rhythms(i.e.,the LR regimen),laying performance and egg quality were well supported whilst significantly decreasing phosphorus consumption and excretion.Thus,serum phosphorus rhythms will need to be carefully maintained when developing dietary phosphorus-reduction strategies in laying hens.