摘要Hepatic lipid and fatty acid(FA)metabolism are critical for regulating energetic homeostasis during embryogenesis.At present,it remains unclear how an exogenous FA intervention affects embryonic development in an avian embryo model.In Exp.1,30 fertilized eggs were sampled on embryonic days(E)16,19,22,25,28,31 and the day of hatch(DOH)to determine the critical period of lipid metabolism.In Exp.2,a total of 120 fertilized eggs were divided into two groups(60 eggs/group)for in ovo feeding(IOF)procedures on E25.Eggs were injected into the yolk sac with PBS as the control group and with oleic acid(OA)as the IOF-OA treatment group.Samples were collected on E28 and E31.In Exp.1,hepatic tri-acylglycerol(TG)and cholesterol(CHO)contents increased while serum TG content decreased from E16 to DOH(P＜0.05).Both serum and liver displayed an increase in unsaturated FA and a decrease in saturated FA(P＜0.05).There was a quadratic increase in the target gene and protein expression related to hepatic FA de novo synthesis and oxidation(P＜0.05),whose inflection period was between E22 and E28.In Exp.2,compared with the control embryos,IOF-OA embryos had an increased yolk sac TG content on E28 and E31,and a decreased serum TG and CHO content on E28(P＜0.05).The IOF-OA embryos had less OA in the yolk sac and liver on E28,and less unsaturated FA in the serum and liver on E31 than did the control embryos(P＜0.05).Hepatic gene mRNA expression related to FA uptake,synthesis,and oxidation on E28 was lower in IOF-OA than in control embryos(P＜0.05),not on E31(P＞0.05).Maximal metabolic changes in lipid and FA metabolism occurred on E22-E28 in Muscovy duck embryogenesis,along with the altered target gene and protein expression related to lipogenesis and lipolysis.IOF-OA intervention on E25 could inhibit the target gene expression related to FA uptake,synthesis,and oxidation,which may influence the normal FA metabolism on E28 during embryogenesis.