摘要Healthy chickens are necessary to meet the ever-increasing demand for poultry meat.Birds are subjected to numerous stressful conditions under commercial rearing systems,including variations in the envi-ronmental temperature.However,it is difficult to counter the effects of global warming on the livestock industry.High environmental temperature is a stressful condition that has detrimental effects on growth and production performance,resulting in decreased feed intake,retarded growth,compromised gut health,enhanced oxidative stress,and altered immune responses.Traditional approaches include nutritional modification and housing management to mitigate the harmful effects of hot environments.Currently,broiler chickens are more susceptible to heat stress(HS)than layer chickens because of their high muscle mass and metabolic rate.In this review,we explored the possibility of in ovo manipulation to combat HS in broiler chickens.Given their short lifespan from hatching to market age,embryonic life is thought to be one of the critical periods for achieving these objectives.Chicken embryos can be modulated through either temperature treatment or nourishment to improve thermal tolerance during the rearing phase.We first provided a brief overview of the harmful effects of HS on poultry.An in-depth evaluation was then presented for in ovo feeding and thermal manipulation as emerging strategies to combat the negative effects of HS.Finally,we evaluated a combination of the two methods using the available data.Taken together,these investigations suggest that embryonic manipulation has the po-tential to confer heat resistance in chickens.