摘要The objectives of this study were to determine the effects of dietary supplementation with citrus flavonoid extracts(CFE)on milk performance,serum biochemistry parameters,fecal volatile fatty acids,fecal microbial community,and fecal metabolites in dairy cows.Eight multiparous lactating Holstein cows were used in a replicated 4×4 Latin square design(21-day period).Cows were fed a basal diet without addition(CON)or basal diet with added CFE at 50(CFE50),100(CFE10),and 150 g/d(CFE150).Feeding CFE up to 150 g/d increased milk yield and milk lactose percentage.Supplementary CFE linearly decreased milk somatic cell count.Serum cytokines interleukin-1β(IL-1 β),IL-2,IL-6,and tumor necrosis factor-α(TNF-α)concentrations decreased linearly as the levels of CFE increased.Cows in CFE150 had lower serum lipopolysaccharide and lipopolysaccharide binding protein compared with CON.These results indicate feeding CFE decreased systemic inflammation and endotoxin levels in dairy cows.Furthermore,feeding CFE linearly increased the concentrations of total volatile fatty acids,acetate,and butyrate in feces.The relative abundances of beneficial bacteria Bifidobacterium spp.,Clostridium coccoides-Eubacterium rectale group,and Faecalibacterium prausnitzii in feces increased linearly with increasing CFE supplementation.The diversity and community structure of fecal microbiota were un-affected by CFE supplementation.However,supplementing CFE reduced the relative abundances of genera Ruminococcus_torques_group,Roseburia,and Lachnospira,but increased genera Bacteroides and Phascolarctobacterium.Metabolomics analysis showed that supplementary CFE resulted in a significant modification in the fecal metabolites profile.Compared with CON,fecal naringenin,hesperetin,hippuric acid,and sphingosine concentrations were greater in CFE150 cows,while fecal GlcCer(d18:1/20:0),Cer(d18:0/24:0),Cer(d18:0/22:0),sphinganine,and deoxycholic acid concentrations were less in CFE150 cows.Predicted pathway analysis suggested that"sphingolipid metabolism"was significantly enriched.Overall,these results indicate that citrus flavonoids could exert health-promoting effects by modulating hindgut microbiome and metabolism in lactating cows.