摘要The objective of this study was to reveal the effect of rumen degradable starch(RDS)on bile acid metabolism and liver transcription in dairy goats using metabolomics and transcriptomics.Eighteen Guanzhong dairy goats of a similar weight and production level(body weight=45.8±1.54 kg,milk yield=1.75±0.08 kg,and second parity)were randomly assigned to 3 treatment groups where they were fed a low RDS(LRDS,RDS=20.52％DM)diet,medium RDS(MRDS,RDS=22.15％DM)diet,or high RDS(HRDS,RDS=24.88％DM)diet,respectively.The goats were fed with the experimental diets for 5 weeks.On the last day of the experiment,all goats were anesthetized,and peripheral blood and liver tissue samples were collected.The peripheral blood samples were used in metabolomic analysis and white blood cell(WBC)count,whereas the liver tissue samples were used in transcriptomic analysis.Based on the metabolomics results,the relative abundances of primary bile acids in the peripheral blood were significantly reduced in the group that was fed the HRDS diet(P＜0.05).The WBC count was significantly increased in the HRDS group compared with that in the LRDS and MRDS groups(P＜0.01),indicating that there was inflammation in the HRDS group.Transcriptomic analysis showed that 4 genes related to bile acid secretion(genes:MDR1,RXRα,AE2,SULT2A1)were significantly downregulated in the HRDS group.In addition,genes related to the immune response were upregulated in the HRDS group,suggesting the HRDS diet induced a hepatic inflammatory response mediated by lipopolysaccharides(LPS)(gene:LBP),activated the Toll-like receptor 4 binding(genes:S100A8,S100A9)and the NF-kappa B signaling pathway(genes:LOC106503980,LOC108638497,CD40,LOC102180880,LOC102170970,LOC102175177,LBP,LOC102168903,LOC102185461,LY96 and CXCL8),triggered inflammation and com-plement responses(genes:C1 QB,C1QC,and CFD).The HRDS diet induced a hepatic inflammatory response may be mediated by activating the Toll-like receptor 4 binding and NF-kappa B signaling pathway after free LPS entered the liver.The changes of bile acids profile in blood and the down-regulation of 4 key genes(MDR1,RXRα,AE2,SULT2A1)involved in bile secretion in liver are probably related to liver inflammation.