摘要Previous studies on porous or nano particles zinc oxide(ZnO)in the piglets have mainly focused on growth performance and intestinal inflammation,but have scarcely explored the efficacy on gut microbiota.In addition,the efficacy of nano particles ZnO,which is related to its product quality,remains undefined.This study aimed to determine the efficacy of dietary 500 mg/kg porous or nano particles ZnO on the growth performance and gut microbiota of the weaned piglets.A total of 128 weaned piglets were randomly assigned to the dietary groups:NC(basal diet),PC(basal diet+3,000 mg/kg conventional ZnO),500HiZ(basal diet+500 mg/kg porous particles ZnO),and 500ZNP(basal diet+500 mg/kg nano particles ZnO).Compared with the NC diet group,both 500HiZ and 500ZNP increased(P＜0.05)average daily feed intake(1 to 28 d)and average daily gain(1 to 28 d),and the 500ZNP tended to decrease feed to gain ratio(F:G ratio,1 to 28 d)(P=0.09).Both 500HiZ and 500ZNP decreased crypt depth of the ileum and increased claudin-2 in the duodenum and zonula occludens-1 in the ileum(P＜0.05).Moreover,both 500HiZ and 500ZNP decreased IL-1p and tumor necrosis factor-α(TNF-α)in the jejunum and decreased TNF-α and IL-6 in the ileum(P＜0.05).Both 500HiZ and 500ZNP increased microbial β-di-versity index in the ileum and microbial α-diversity indices in the colon of piglets(P＜0.05).The pro-biotic genera Coprococcus(500ZNP)and Blautia(500HiZ)were positively correlated with the F:G ratio(1 to 28 d)in colon of piglets(P＜0.05).In addition,500HiZ promoted mitochondrial fusion protein 1(MFN1)and zinc transporter-1(ZnT-1)in the jejunum(P＜0.05),whilst 500ZNP decreased MFN1 in the jejunum and ZnT-1 in the ileum(P＜0.05).In summary,both 500HiZ and 500ZNP improved the growth performance of piglets,which is likely via the genera Blautia and Coprococcus,respectively.Both 500HiZ and 500ZNP improved barrier function and inflammation of the intestine,and 500HiZ achieved better efficacy than 500ZNP on intestine mitochondrial functions.