摘要The compromised egg quality and leg abnormality during the end of the laying cycle(after 40 weeks)have been leading to poor animal welfare and substantial economic losses.Therefore,the effects of fermented calcium(Ca)butyrate,produced by fermentation by Clostridium butyricum,on production,eggshell quality,and tibial property of hens were explored.A total of 192 Hy-line brown laying hens at 50-week-old were assigned to a basal diet or the basal diet with 300 mg/kg of the fermented Ca butyrate from 50 to 58 weeks of age.Each treatment had 6 replicates with 16 hens each.The diet supplemented with 300 mg/kg fermented Ca butyrate notably increased egg weight,ovarian follicle number,and eggshell strength(P=0.072)as compared to the basal diet,which were associated with cytokine secretion,toll-like receptor signaling pathways,and intestinal immunity based on the RNA-seq data from the granulosa.Dietary Ca butyrate inclusion decreased the expression of ileal tumor necrosis factor-alpha and serum pro-inflammatory cytokine concentration,as well as increased the content of serum immunoglobulin A when compared to the basal diet(both P＜0.05).The birds that received fermented Ca butyrate diets exhibited higher villus height(P＜0.05)and upregulated expression of tight junction proteins,whereas it did not alter the composition of cecal microbiota(P＞0.05).In addition,the diet with fermented Ca butyrate reduced the number of osteoclasts in the proximal tibia and the level of C-ter-minal cross-linked telopeptide of type Ⅰ collagen,a bone resorption marker(P＜0.05),whereas it tended to increase the concentration of the procollagen type Ⅰ N-terminal propeptide that reflects bone for-mation marker in serum.Moreover,the layers fed fermented Ca butyrate diets possessed higher(P＜0.05)bone area and trabecular number of the proximal tibia,yield load,and ultimate load than those that consumed basal diets.Collectively,dietary fermented Ca butyrate supplementation in post-peak layer diets improved the ovarian function and tibia quality,which might be related to enhancing in-testinal integrity and consequently decreasing inflammation mediated bone resorption.