摘要The present study was to evaluate the effect of trace minerals(Zn,Mn,and Cu)from complexed amino acid minerals(ZMCAA)and bis-glycinate chelated minerals(ZMCGly)in laying hen diets on performance,internal and external egg quality,yolk mineral deposition,intestinal morphometry,and bone charac-teristics.From 78 to 98 weeks of age,400 White LSL-Lite strain laying hens were distributed in a ran-domized design with 4 treatments with 10 replicates per treatment.Treatments were distributed in a 2 x 2 factorial arrangement using either Zn,Mn,and Cu of ZMCAA or ZMCGly source at 2 levels:low(20,20,and 3.5 mg/kg of Zn,Mn,and Cu,respectively)or high(40,40,and 7 mg/kg of Zn,Mn,and Cu,respectively).The analysis of variance was performed,and in cases where differences were observed,the means were compared using Tukey's test(P＜0.05).The source and level of trace mineral supplemen-tation had a significant impact on the performance of laying hens.Hens fed ZMCAA had higher egg production(P=0.01),egg weight(P=0.02),egg mass(P=0.01),and lower feed conversion ratio(P=0.05)compared to those fed ZMCGly.The ZMCAA supplementation showed higher albumen height(P=0.01),albumen weight(P=0.01),and eggshell thickness(P＜0.01).The deposition of Zn(P＜0.01),Mn(P＜0.01),and Cu(P＜0.01)in the egg yolk was greater for hens received ZMCAA.Tibia weight(P=0.04)and bone densitometry(P＜0.01)in the tibia were higher with ZMCAA supplementation.In the small intestine,ZMCAA resulted in longer villi(P=0.02)and shorter crypt depth(P=0.01)in the duodenum.Jejunum and ileum measurements were influenced by the level and source of trace minerals(P＜0.05).Laying hens fed ZMCAA exhibited superior performance,egg quality,deposition of trace minerals in the egg yolk,and bone density compared to hens fed ZMCGly.In this study,older laying hens supplemented with ZMCAA at lower levels demonstrated adequate levels of supplementation.