摘要This review explores the potential of including glutamine,a so-called non-essential amino acid,in the formulation of reduced-crude protein(CP)diets for broiler chickens.There is a precedent for benefits when including glycine and serine in reduced-CP diets.Fundamentally this is due to decreases in non-essential amino acid concentrations in reduced-CP diets-an unavoidable consequence of reducing CP without amino acid supplementation.The situation for glutamine is complicated because analysed dietary concentrations are very rarely provided as standard assays do not differentiate between gluta-mine and glutamate and are reported on a combined basis as glutamic acid.The dietary requirement for glutamic acid is approximately 36.3 g/kg but it is increasingly unlikely that this requirement will be met as dietary CP levels are progressively reduced.Glutamine is an abundant and versatile amino acid and constitutes 50.5 mg/g of whole-body chicken protein and is the dominant free amino acid in systemic plasma where it has been shown to provide 22.6％(139.9 of 620.3 μg/mL)of the total in birds offered 215 g/kg CP,wheat-based diets.In addition to dietary intakes,glutamine biosynthesis is derived mainly from the condensation of glutamate and ammonia(NH3)catalysed by glutamine synthetase,a reaction that is pivotal to NH3 detoxification.Glutamate and NH3 are converted to glutamine by phosphate-dependent glutaminase in the reciprocal reaction;thus,glutamine and glutamate are interchangeable amino acids.However,the rate of glutamine biosynthesis may not be adequate in rapidly growing broiler chickens and exogenous and endogenous glutamine levels are probably insufficient in birds offered reduced-CP diets.The many functional roles of glutamine,including NH3 detoxification and maintenance of acid-base homeostasis,then become relevant.Twenty feeding studies were identified where dietary glutamine supplementation,usually 10 g/kg,was evaluated in birds kept under thermoneutral condi-tions.On balance,the outcomes were positive,but the average dietary CP was 213 g/kg across the twenty feeding studies,which indicates that CP and,in turn,glutamine concentrations would have been adequate.This suggests that glutamine inclusions in reduced-CP diets hold potential and consideration is given to how this may be best confirmed.