摘要The intracellular lipids in muscle cells of farm animals play a crucial role in determining the overall intramuscular fat(IMF)content,which has a positive impact on meat quality.However,the mechanisms underlying the deposition of lipids in muscle cells of farm animals are not yet fully understood.The purpose of this study was to determine the roles of carbohydrate-response element binding protein(ChREBP)and fructose in IMF deposition of chickens.For virus-mediated ChREBP overexpression in tibialis anterior(TA)muscle of chickens,seven 5-d-old male yellow-feather chickens were used.At 10 d after virus injection,the chickens were slaughtered to obtain TA muscles for analysis.For fructose administration trial,sixty 9-wk-old male yellow-feather chickens were randomly divided into 2 groups,with 6 replicates per group and 5 chickens per replicate.The chickens were fed either a basal diet or a basal diet supplemented with 10％fructose(purity ≥ 99％).At 4 wk later,the chickens were slaughtered,and breast and thigh muscles were collected for analysis.The results showed that the skeletal ChREBP mRNA levels were positively associated with IMF content in multiple species,including the chickens,pigs,and mice(P＜0.05).ChREBP overexpression increased lipid accumulation in both muscle cells in vitro and the TA muscles of mice and chickens in vivo(P＜0.05),by activation of the de novo lipo-genesis(DNL)pathway.Moreover,activation of ChREBP by dietary fructose administration also resulted in increased IMF content in mice and notably chickens(P＜0.05).Furthermore,the lipidomics analysis revealed that ChREBP activation altered the lipid composition of chicken IMF and tented to improve the flavor profile of the meat.In conclusion,this study found that ChREBP plays a pivotal role in mediating the deposition of fat in chicken muscles in response to fructose-rich diets,which provides a novel strategy for improving meat quality in the livestock industry.