摘要Heat stress poses a significant threat to the global livestock industry,particularly impacting dairy cows due to their higher metabolic heat production and increased susceptibility.The rumen microbiota plays a crucial role in regulating heat stress in dairy cows.Moreover,the rumen-mammary gland axis has been recently unveiled,indicating that rumen bacteria and their metabolites can influence mammary gland health and function.Extracellular vesicles,cell-derived vesicles,are known to carry various biomolecules and mediate intercellular communication and immune modulation.This review proposes the hypothesis that heat stress poses a threat to dairy cows via the rumen-mammary gland axis by regulating rumen microbiota and their secreted extracellular vesicles.It summarizes existing knowledge on bacterial extracellular vesicles and the rumen-mammary gland axis,suggesting that targeting the rumen micro-biota and their extracellular vesicles,while enhancing mammary gland health through this axis,could be a promising strategy for preventing and alleviating heat stress in dairy cows.The aim of this review is to offer new insights and guide future research and development efforts concerning heat stress in dairy cows,thereby contributing to a deeper understanding of its pathogenesis and potential interventions.