摘要Dietary sanguinarine(SAN)can enhance the growth performance of poultry and livestock,but the regulatory mechanism of the SAN monomer on intestinal homeostasis and how it promotes growth performance has not yet been clarified.In this study,200 chickens were divided into four groups and fed different doses of SAN(0,0.225,0.75,2.25 mg/kg)for transcriptome and microbiota analysis.The data showed that different doses of SAN supplementation increased the feed conversion rate(FCR)of 22 to 42 d old and 1 to 42 d old broilers(P＜0.01),and 0.225 mg/kg SAN reduced the contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST),creatinine(CREA)and blood urea nitrogen(BUN)in serum(P＜0.01).Dietary SAN increased the villus height and the villus height/crypt depth(V/C)ratio in the ileum(P＜0.01).The levels of tight junction proteins(zonula occludens-1,occludin and claudin-1)were up-regulated in the ileum and cecum(P＜0.01)and the levels of immunoglobulin(Ig)A,IgM,IgG,interleukin(IL)-4,IL-10 and interferon(IFN)-y were up-regulated in the serum and ileum(P＜0.01).RNA-seq analysis revealed 385 differentially expressed genes(DEGs)(|log2 fold change|≥ 1,FDR＜0.05)between the SAN group and CON group.Kyoto Encyclopedia of Genes and Genomes pathway analysis showed 15 pathways mostly associated with the immune system.Additionally,the reverse transcription-PCR results showed that the relative mRNA expression of β-defensin and mucin 2 were up-regulated(P＜0.01)and Toll-like receptor(TLR2 and TLR4)mRNA expression were down-regulated by SAN(P＜0.01),which was consistent with the transcriptomic analysis.Western blot analysis also showed that SAN reduced the expression of inflammatory proteins such as TLR4,nuclear factor-kappa B and IL-1βin the ileum(P＜0.01).In addition,at the genus level,SAN significantly increased the relative abundance of bacteria(Bacteroides,unclassified_f_Lachnospiraceae,Lactobacillus and Romboutsia)involved in acetate and butyrate production in the cecum,which are associated with enhanced intestinal immune function and maintaining intestinal health.In conclusion,SAN ameliorates the growth performance of broilers,enhances intestinal immune function,regulates the structure of microbiota and maintains intestinal health.