摘要During the growing period,the gastrointestinal tract of layer pullets is not yet well developed and may be susceptible to dietary energy level.The energy level and composition might impact the intestinal energy metabolism of layer pullets.To test this hypothesis,a total of 480"Jing Tint 6"layer pullets were used in an 8-week study and allocated to 4 groups,each consisting of 8 replicates,with 15 birds per replicate.Pullets were treated with low or high starch:fat ratios(LS,10∶1;HS,20∶1)in a 2 × 2 factorial arrangement with regular energy(RE,11.85 and 11.68 MJ/kg for birds from 6 to 10 weeks of age and 11-14 weeks of age,respectively)or low energy(LE,0.55 MJ/kg lower than RE)levels.A significant interaction(P＜0.05)showed that HS increased glandular stomach weight and the jejunal villus length to crypt depth ratio(VCR)in LE diets,but decreased these parameters in RE diets.Dietary energy reduction impaired energy metabolism in the ileum(P＜0.05)mainly via decreasing the gene expression of en-zymes involved in the tricarboxylic acid(TCA)cycle(α-ketoglutarate dehydrogenase complex[α-KGDH];isocitrate dehydrogenase(NAD(+)[IDH]catalytic;citrate synthase[CS])and adenosine triphosphate(ATP)synthesis,reducing contents of phosphoenolpyruvate(PEP)and adenylate energy charges(AEC)and down-regulating the adenosine monophosphate-activated protein kinase(AMPK)pathway.HS stimulated AMPKα phosphorylation,increased protein abundance of peroxisome proliferator activated-receptor gamma coactivator 1α(PGC1α)and improved contents of amino acids(aspartate,glutamate,glutamine,alanine and threonine)and malate in the ileum regardless of energy levels(P＜0.05).By an interaction(P＜0.05),the transition from LS to HS diets up-regulated ileal gene expression of AMPKα1 and decreased content of adenosine monophosphate(AMP),accompanied by higher AEC but only in birds fed with LE diets.Collectively,these results suggest that low energy feeding may not be enough for maintaining intestinal energy homeostasis in layer pullets and emphasizes the importance of a relatively high starch:fat ratio in restoring energy metabolism in the ileum.