摘要The current study aims to investigate the potential interaction between glycosylation profiles of the Ningxiang breed(NX)and Western Duroc × Landrace × Yorkshire breed(DLY)weaned piglets,and their characteristic microbes,employing integrated analyses of transcriptomics and metagenomics.Twenty-four(12 NX and 12 DLY)at 28 days of age were transported into an experimental house and fed the same weaned piglet diet.The trail period was 7 days.Results revealed that the NX piglets had a higher growth-to-feed ratio,body weight gain scale,and lower pathological score of intestinal injury compared with the DLY piglets(P＜0.01).DLY piglets displayed elevated mRNA expression levels of MUC2 and MUC5AC in colonic mucosal tissue than NX piglets(P＜0.05).Within the O-linked glycosylated differen-tially expressed genes(DEGs),FNTA,GALNT18,POMGNT1,POMGNT2,and POMT1 were significantly upre-gulated in DLY piglets relative to NX piglets(P＜0.05).Conversely,C1GALT2,GALNT1,KMT2C,and OGT were significantly downregulated in DLY piglets compared to NX piglets(P＜0.05).The KMT2C gene was hardly expressed in the transcriptome of DLY piglets.At the phylum taxonomic level,NX piglets had a higher abundance of Firmicutes,while DLY piglets had a higher abundance of Proteobacteria.At the genus taxonomic level,NX piglets had a higher abundance of Lactobacillus,whereas DLY piglets had a higher abundance of Collinsella,Enterococcus and Escherichia.The results of the correlation between intestinal differential bacteria and O-chain glycosylated DEG showed that C1GALT2,GALNT1 and KMT2 were asso-ciated with Lactobacillus_pontis showed a positive correlation(R=0.67).Through comparative analysis of differentially glycosylated genes and their associated functions,this study highlights the potential role of reduced expression of GALNT1 and KMT2C genes,involved in O-linked protein and glycan reactions,in impairing the intestinal barrier function of DLY piglets.Furthermore,members of the Lactobacillus and Prevotella genera may actively contribute to the regulation of piglet colon glycosylation profiles.