摘要A substantial amount of Earth's water is inadequate for human consumption while local demand is outstripping traditional supplies in many world regions;thereby,brackish and seawater treatment has become a prerequisite.This investigation suggested a complete design of an RO-based desalination filter with a multilayer biomimetic membrane.The study dem-onstrated a comprehensive method for experimentally fabricating a proprietary biomaterial-based multilayer nano-porous membrane.This analysis revealed that Silk Nano-Fibril(SNF)and Hydroxyapatite(HAP)extracted from Bombyx Mori silkworm cocoons may be utilized to manufacture highly methodical multilayer membranes by incorporating protein-self-assembly and in-situ-bio-mineralization.Membrane's aquaporin layer containing lipid-bilayers has rapid water permeability and high efficacy at eliminating salt ions and contaminants.The 4 μm thick SNF/HAP membrane showed a considerable decrease in salinity,with a salt rejection of 93.33％.The proposed membrane had a saline water permeability of 6.58 LMH/bar,almost 61.09％higher than conventional TFC membranes.Hydrophobic barrier and spiral-wrapped filter architecture of the membrane enable low fouling and self-cleaning properties.The schematic filter design and biomimetic fabrication of the SNF/HAP membrane have formulated a conceptual framework that might direct to the broad-scale,low-cost RO water purification filters,increasing the efficiency of water desalination and boosting the effectiveness of water treatment technolo-gies to reduce potable water scarcity.