摘要Osteomyelitis caused by Staphylococcus aureus(S.aureus)is a severe inflammatory bone disease that is difficult to eradi-cate and can be life-threatening.Traditional treatments relying on high-dose systemic antibiotics often fail due to biofilm resistance and emerging drug-resistant strains.This study proposes a diflunisal-loaded 3D-printed scaffold as a novel thera-peutic strategy.A composite biomaterial ink composed of gelatin(Gel),polycaprolactone(PCL),and nanohydroxyapatite(n-HA)was synthesized and used to fabricate customized porous scaffolds via 3D printing.Diflunisal was loaded onto the scaffolds using a Gel swelling method.In vitro experiments showed sustained diflunisal release under different pH conditions(pH=6.0 and 7.4)mimicking infection and protection of bone marrow stromal cells(BMSCs)from S.aureus toxins.In vivo studies revealed significant alleviation of infection and promotion of bone regeneration at the defect site.This diflunisal-loaded Gel/PCL/n-HA scaffold integrates anti-virulence therapy with bone regeneration,offering a promis-ing solution for osteomyelitis treatment.