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Genomic,transcriptomic,and metabolomic analyses reveal convergent evolution of oxime biosynthesis in Darwin's orchid

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摘要Angraecum sesquipedale,also known as Darwin's orchid,possesses an exceptionally long nectar spur.Charles Darwin predicted the orchid to be pollinated by a hawkmoth with a correspondingly long proboscis,later identified as Xanthopan praedicta.In this plant-pollinator interaction,the A.sesquipedale flower emits a complex blend of scent compounds dominated by diurnally regulated oximes(R1R2C=N-OH)to attract crepuscular and nocturnal pollinators.The molecular mechanism of oxime biosynthesis remains un-clear in orchids.Here,we present the chromosome-level genome of A.sesquipedale.The haploid genome size is 2.10 Gb and represents 19 pseudochromosomes.Cytochrome P450 encoding genes of the CYP79 family known to be involved in oxime biosynthesis in seed plants are not present in the A.sesquipedale genome nor the genomes of other members of the orchid family.Metabolomic analysis of the A.sesquipedale flower revealed a substantial release of oximes at dusk during the blooming stage.By inte-grating metabolomic and transcriptomic correlation approaches,flavin-containing monooxygenases(FMOs)encoded by six tandem-repeat genes in the A.sesquipedale genome are identified as catalyzing the formation of oximes present.Further in vitro and in vivo assays confirm the function of FMOs in the oxime biosynthesis.We designate these FMOs as orchid oxime synthases 1-6.The evolutionary aspects related to the CYP79 gene losses and neofunctionalization of FMO-catalyzed biosynthesis of oximes in Dar-win's orchid provide new insights into the convergent evolution of biosynthetic pathways.

作者 Kai Jiang ^[1] Birger Lindberg M?ller ^[2] Shaofan Luo ^[3] Yu Yang ^[4] David R.Nelson ^[5] Elizabeth Heather Jakobsen Neilson ^[2] Joachim M?ller Christensen ^[2] Kai Hua ^[1] Chao Hu ^[1] Xinhua Zeng ^[1] Mohammed Saddik Motawie ^[2] Tao Wan ^[6] Guang-Wan Hu ^[6] Guy Eric Onjalalaina ^[7] Yijiao Wang ^[3] Juan Diego Gaitán-Espitia ^[8] Zhiwen Wang ^[9] Xiao-Yan Xu ^[10] Jiamin He ^[11] Linying Wang ^[12] Yuanyuan Li ^[12] Dong-Hui Peng ^[12] Siren Lan ^[12] Huiming Zhang ^[13] Qing-Feng Wang ^[6] Zhong-Jian Liu ^[12] Wei-Chang Huang ^[14] 学术成果认领

作者单位 Shanghai Key Laboratory of Plant Functional Genomics and Resources,CAS Center for Excellence in Molecular Plant Sciences Chenshan Plant Science Research Center,Shanghai Chenshan Botanical Garden,Shanghai 201602,China;Eastem China Conservation Centre for Wild Endangered Plant Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602,China ^[1] Plant Biochemistry Laboratory,Department of Plant and Environmental Science,University of Copenhagen,Copenhagen,Denmark;VILLUM Research Center for Plant Plasticity,University of Copenhagen,Copenhagen,Denmark ^[2] Shanghai Key Laboratory of Plant Functional Genomics and Resources,CAS Center for Excellence in Molecular Plant Sciences Chenshan Plant Science Research Center,Shanghai Chenshan Botanical Garden,Shanghai 201602,China ^[3] Shanghai Center for Plant Stress Biology,Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 201602,China ^[4] Department of Microbiology,Immunology and Biochemistry,University of Tennessee Health Science Center,Memphis,TN,USA ^[5] State Key Laboratory of Plant Diversity and Specialty Crops,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan,China;Sino-Africa Joint Research Center,Chinese Academy of Sciences,Wuhan,China ^[6] Sino-Africa Joint Research Center,Chinese Academy of Sciences,Wuhan,China;University of Antananarivo,Antananarivo,Madagascar ^[7] The Swire Institute of Marine Science and School of Biological Sciences,The University of Hong Kong,Hong Kong SAR,China ^[8] PubBio-Tech,Wuhan,China ^[9] National Key Laboratory of Plant Molecular Genetics,Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China ^[10] Shanghai Key Laboratory of Plant Functional Genomics and Resources,CAS Center for Excellence in Molecular Plant Sciences Chenshan Plant Science Research Center,Shanghai Chenshan Botanical Garden,Shanghai 201602,China;Key Laboratory of Orchid Conservation and Utilization of National Forestry and Grassland Administration at College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,China ^[11] Key Laboratory of Orchid Conservation and Utilization of National Forestry and Grassland Administration at College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,China ^[12] Shanghai Center for Plant Stress Biology,Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 201602,China;Key Laboratory of Plant Design,Chinese Academy of Sciences,Shanghai 200032,China ^[13] Shanghai Key Laboratory of Plant Functional Genomics and Resources,CAS Center for Excellence in Molecular Plant Sciences Chenshan Plant Science Research Center,Shanghai Chenshan Botanical Garden,Shanghai 201602,China;Eastem China Conservation Centre for Wild Endangered Plant Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602,China;Key Laboratory of Orchid Conservation and Utilization of National Forestry and Grassland Administration at College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,China ^[14]

关键词 Angraecum sesquipedale whole genome sequence white floral scent convergent evolution flavin monooxygenase CYP79 absence

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Research articles

DOI 10.1016/j.molp.2024.12.010

发布时间 2025-05-26（万方平台首次上网日期，不代表论文的发表时间）

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