摘要目的 了解广东省东莞市镇区7岁以下小儿常见呼吸道病毒感染的病原学特点及其影响因素.方法 收集2007年2-10月东莞市石碣医院儿科门诊及病房7岁以下小儿急性呼吸道感染病例的呼吸道分泌物标本340例,采用逆转录-聚合酶链反应法(RT-PCR)检测呼吸道合胞病毒A、B型(RSV-A、B型),流感病毒甲、乙型(FLU-A、B型),副流感病毒1、2、3型(PIV-1、2、3型)和人偏肺病毒(hMPV).结果 从340例呼吸道分泌物标本中检测出常见呼吸道病毒171例,阳性率为50.29%;其中RSV-A型69例(20.29%),FLU-A型39例(11.47%),hMPV 32例(9.41%),PIV3型22例(6.47%),RSV-B型9例(2.65%).在171例呼吸道病毒阳性病例中,有混合病毒感染16例,占所有病毒感染者9.36%;其中hMPV合并RSV-A感染者5例,RSV-A合并PIV-3者4例,hMPV合并FLU-A、PIV-3感染者各2例,FLU-A合并PIV-3者2例,RSV-A合并FLU-A者1例;但未见三重混合感染或多重感染.未榆测出FLU-B型及PIV-1,2型.结论 呼吸道病毒是东莞镇区7岁以下小儿急性呼吸道感染的重要病原体,RSV为最主要病原,其次为FLU-A和hMPV.

abstractsObjective To study etiological characteristics of common respiratory viruses infection in children under 7 years old in Dongguan city of Guangdong province.Methods The total RNA were extracted from 340 specimens which had been collected from children under 7 years old during February 2007 to October 2007 in Shijie hospital.During the study the respiratory viruses including respiratory syncytial virus A and B(RSV-A and B), influenza A and B(FLU-A and B), parainfluenza 1, 2, 3(PIV-1, 2 and 3) and Human metapneumo virus (hMPV) were detected by reverse transcription-polymerase chain reaction(RT-PCR).Results The common respiratory viruses were demonstrated in 340 children as the positive 50.29%(171/340),including 69 cases (20.29%) with RSV-A infection, 39 cases(11.47%) with FLU-A, 32 cases (9.41%) with hMPV, 22 cases (6. 47%) with PIV-3,9 cases (2.65%) with RSV-A, and 16 cases (9.36% of all viral infection positive cases) with the mixed infection.In these respiratory viruses mixed infection cases,there were 5 hMPV cases coinfected with RSV-A, 4 RSV-A cases with PIV-3, 2 hMPV eases with PIV-3, 2 hMPV cases with FLU-B, 2 cases FLU-A with PIV-3, and 1 ease RSV-A with FLU-A, FLU-B, PIV-1, PIV-2, and multiple mixed infections haven't been detected in this study.Conclusions The data show common respiratory viruses are significant pathogens in acute respiratory infection of paediatric patients under 7 years old in Dongguan city.RSV is still the leading causes of acute respiratory infection in children, the next pathogens respectively are FLU-A and hMPV.