摘要目的 用泛耐药鲍曼不动杆菌经腹腔注射小鼠,观察及评估其感染情况,建立小鼠急性感染模型.方法 用不同浓度泛耐药鲍曼不动杆菌注射小鼠,以生理盐水为阴性对照,同时以铜绿假单胞菌ATCC 27853为质控对照.观察小鼠的生存状况,并在不同时间检测小鼠血液中CRP浓度及白细胞计数,计算LD50,最后培养及鉴定模型小鼠病灶中的细菌菌种.结果 小鼠经腹腔注射不同浓度的泛耐药鲍曼不动杆菌后,呈感染表现;白细胞与C反应蛋白在小鼠感染6h后呈现不同程度的升高,并与对照组比较差异有统计学意义.3×109 cfu/ml组小鼠最先表现临床症状和死亡,最短死亡时间为接种后12h,死亡率达100％,经计算小鼠模型的LD50为1.5×108 cfu/ml.经培养鉴定,从感染死亡的小鼠分离的菌株与接种菌一致.结论 泛耐药鲍曼不动杆菌诱发小鼠细菌性的急性感染时,呈现人感染该菌后的部分症状,可利用此模型进行临床的实验研究工作.

abstractsObjective To establish mouse models with acute infection by intraperitoneally injecting pan-drug-resistant Acinetobacter Baumannii and then to observe and assess the infectious status.Methods Different concentrations of pan-drug-resistant Acinetobacter baumannii were injected into mice.Ones injected saline were set as negative controls and Pseudomonas aeruginosa(ATCC 27853) as quality controls.The living conditions of the mice were observed.The CRP levels and white blood cell count at different times were detected.The LD50 was calculated.Finally,the bacterial species were cultured and identified in the mouse models.Results The mice were intraperitoneally injected with different concentrations of pan-drug-resistant Acinetobacter Baumannii and showed signs of infection.6 hours after infection,the white blood cells and C-reactive protein level in the mice increased in varying degrees and were statistically different from those in the control group.Clinical symptoms and death first occurred in the 3 × 109 cfu / ml group,with a shortest time from inoculation to death of 12 h and a mortality rate of 100％.The calculation showed that the LD50 of the mouse models was 1.5 × 108 cfu / ml.The bacterial culture showed that the bacteria separated from the dead infected mice were consistent with the inoculants.Conclusions Pan-drug-resistant Acinetobacter baumannii can induce acute bacterial infections in mice and shows the similar symptoms of people infected with the bacteria,so this mouse model can be use in experimental clinical studies.