摘要The purpose of vaccination is to generate Neutralizing antibodies(NAbs),as these antibodies have neutralizing activ-ity against viral infections such as HIV and SARS-CoV-2.NAb has high antiviral potency and therefore be served as therapeutics.However,induction of NAb is not easy,and RNA viruses such as HIV and SARS-CoV-2 always escape NAb binding epitopes due to the rapid mutation of their genomes.Thus,despite Fab and neutralizing,other compo-nent and its function against viral infection is important in the biology of antibodies,such as the Fc part in antibodies.Fc-mediated function showed protective potency in viral infection.Fc-mediated ADCC contributes to protect against HIV in phase II/III clinical trial,but remains controversy.Marginal evidences show protectivity of non-NAb and Fc-mediated ADCC in HIV infection and AIDS progress.In SARS-CoV-2 infection,Fc-mediated ADCC showed po-tent antiviral activity as well.While,the participation of non-NAb is elusive in the control of HIV and SARS-CoV-2 dual infection.Limited data reported that Fc-mediated functions is regulated following HIV infection as people living with HIV after vaccination of pulmonary pathogens display increased ADCC activity to homologues pathogens.In order to reveal Fc-mediated function,there is a need to actively develop a robust and accurate in vitro assay to mea-sure it.This is an emerging attract that have drawn much attention.