摘要目的 探讨HIV-1辅助受体基因预测工具对我国流行的CRF01_AE毒株辅助受体利用的可行性.方法 从北京市、广西壮族自治区、四川省的HIV-1感染者中分离出CRF01_AE毒株22株,利用基因型方法(11/25规则、WebPSSM和geno2pheno)对病毒辅助受体利用情况进行预测,将预测结果与经GHOST细胞系检测的表型实验结果进行比较.结果 经GHOST细胞系检测,22株CRF01_ AE毒株中,4株利用CCR5/CXCR4辅助受体,18株利用CCR5辅助受体.11/25规则预测与表型实验的一致率为86.4％;geno2pheno中,G2p-clinical、NGS-sanger和G2p-colon模型预测与表型实验的一致率分别为95.5％、86.4％和68.2％;WebPSSM中,PSSM-sinsi_B、PSSM-x4r5和PSSM-sinsi_C模型预测与表型实验的一致率分别为86.4％、72.7％和45.5％.结论 对于我国CRF01_AE毒株的HIV-1感染者,在使用辅助受体拮抗剂之前应进行辅助受体利用的预测,Geno2pheno是较准确的辅助受体基因预测工具.

abstractsObjective To analyze feasibility of using genotypic prediction for coreceptor usage of HIV-1 CRF01_AE strains circulating in China.Methods Twenty-two CRF01_AE strains were isolated from HIV-1 infected patients in Beijing,Guangxi and Sichuan.The genotypic methods (11/25 rule,WebPSSM and geno2pheno) were used to predict coreceptor usage of the strains.The prediction were compared with the results of phenotypic testing based on GHOST cell line.Results Of the 22 CRF01_AE strains,4 strains used CCR5/CXCR4 coreceptor and 18 strains used the CCR5 coreceptor,as detected by the GHOST cell line test.The consistency rate between genotypic prediction by 11/25 rule and phenotypic test was 86.4％.For geno2pheno,the consistency rates between genotypic predictions by G2p-clinical,NGS-sanger and G2p-colon models and phenotypic tests were 95.5％,86.4％ and 68.2％,respectively.For WebPSSM,the consistency rates between genotypic predictions by PSSM-sinsi_B,PSSM-x4r5,and PSSM-sinsi_C models and phenotypic tests were 86.4％,72.7％ and 45.5％,respectively.Conclusions For the patients infected with HIV-1 CRF01_AE strains in China,coreceptor usage should be predicted before treatment of coreceptor antagonists.Geno2pheno is a more accurate way for genotypic prediction of HIV coreceptor usage.